Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB120-6581 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-6581, RRID:AB_789359
- Product name
- Rabbit Polyclonal Collagen IV alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Conjugate
- Biotin
- Antigen sequence
The development of type-specific an
tibodies is dependent on NON-DENATU
RED three-dimensional epitopes.- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Submitted references Functional and phenotypic distinction of the first two trophoblast subdivisions and identification of the border between them during early postimplantation: A prerequisite for understanding early patterning during placentogenesis.
Nikolaou S, Hadjikypri X, Ioannou G, Elia A, Georgiades P
Biochemical and biophysical research communications 2018 Jan 29;496(1):64-69
Biochemical and biophysical research communications 2018 Jan 29;496(1):64-69
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [Biotin] [NB120-6581] - collagen IV antibody 1:400, 45 min RT showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody [Biotin] [NB120-6581] - Immunohistochemical staining of Collagen IV alpha 1 (red) and Insulin (green) in mouse pancreas. Image from verified customer review.