Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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Comment
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- Product number
- R1041 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-COL4A1 Antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-COL4A1 Antibody, Aff - Purified
- Reactivity
- Human, Bovine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.1mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
Submitted references Perlecan is critical for heart stability.
Sasse P, Malan D, Fleischmann M, Roell W, Gustafsson E, Bostani T, Fan Y, Kolbe T, Breitbach M, Addicks K, Welz A, Brem G, Hescheler J, Aszodi A, Costell M, Bloch W, Fleischmann BK
Cardiovascular research 2008 Dec 1;80(3):435-44
Cardiovascular research 2008 Dec 1;80(3):435-44
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Collagen IV antibody (1/400, 45 min RT) showed strong staining in FFPE sections of Human kidney (Left) with strong Red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA
- Validation comment
- IHC