Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C745526 - Provider product page
- Provider
- LSBio
- Product name
- COL4A1 / Collagen IV Alpha1 Antibody (HRP) LS-C745526
- Antibody type
- Polyclonal
- Reactivity
- Human, Bovine
- Host
- Rabbit
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Storage
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
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Supportive validation
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- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of rabbit Anti-Collagen IV Biotin Conjugated Antibody. Tissue: human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: Collagen IV antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen IV is extracellular. Staining: Collagen IV as precipitated red signal with hematoxylin purple nuclear counterstain.