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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-85634 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COL4A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (86%), Rat (90%), Bovine (82%). Positive Control: SK-N-SH. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.32 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Collagen IV in whole cell extract using Collagen IV polyclonal antibody (Product # PA5-85634) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using COL4A1 Polyclonal Antibody (Product # PA5-85634). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 Polyclonal Antibody (Product # PA5-85634) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Collagen IV in paraffin-embedded mouse brain using Collagen IV polyclonal antibody (Product # PA5-85634) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COL4A1 Polyclonal Antibody detects COL4A1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. COL4A1 stained by COL4A1 Polyclonal Antibody (Product # PA5-85634) diluted at 1:2,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
