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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [9]
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Validation data
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- Product number
- LS-C175525 - Provider product page
- Provider
- LSBio
- Product name
- ABCB1 / MDR1 / P Glycoprotein Antibody (clone OTI2C7) LS-C175525
- Antibody type
- Monoclonal
- Description
- Protein A/G purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2C7
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or fragment (995-1280 AA) of ABCB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCB1. .
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of extracts (15 ug) from 4 different cell lines by using anti-ABCB1 monoclonal antibody (Lane 1: 293T; Lane 2: UO_31; Lane 3: HCT_115; Lane 4: HeLa).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Human Kidney tissue using anti-ABCB1 mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ABCB1 mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-ABCB1 mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Human pancreas tissue using anti-ABCB1 mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ABCB1 mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ABCB1 mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ABCB1 mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-ABCB1 mouse monoclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- IHC of paraffin-embedded Human pancreas tissue using anti-ABCB1 mouse monoclonal antibody.
