Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B1448 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B1448, RRID:AB_2220662
- Product name
- IHC-plus™ ABCB1 / MDR1 / P Glycoprotein Antibody LS-B1448
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Aliquot and store at -20°C or below for extended storage. Avoid freeze / thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.
Submitted references miR-200c inhibits melanoma progression and drug resistance through down-regulation of BMI-1.
Liu S, Tetzlaff MT, Cui R, Xu X
The American journal of pathology 2012 Nov;181(5):1823-35
The American journal of pathology 2012 Nov;181(5):1823-35
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-ABCB1 Antibody - Western Blot. Western blot of affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.