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LearnAP09346PU-N
antibody from Acris Antibodies GmbH
Targeting: ABCB1
ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- AP09346PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09346PU-N, RRID:AB_2035287
- Product name
- anti CD243 / MDR1 (262-277)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to amino acids 262-277 of Human ABCB1 protein.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blot using affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells overexpressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10,000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.
