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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA809814 - Provider product page
- Provider
- OriGene
- Product name
- ABCB1 mouse monoclonal antibody,clone OTI9C10
- Antibody type
- Monoclonal
- Description
- ABCB1 mouse monoclonal antibody,clone OTI9C10
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ABCB1
- Isotype
- IgG
- Antibody clone number
- OTI9C10
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCB1 (RC216080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCB1.(1:2000)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABCB1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809814)(1:2000)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809814)(1:2000)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of permeabilized Hek293T cells, using anti-ABCB1 antibody(TA809814),(Red), compared to negative control (PBS),(Blue).(1:100).
- Validation comment
- FC
