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LearnNBP2-52654-0.2mg
antibody from Novus Biologicals
Targeting: ABCB1
ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1
Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- NBP2-52654-0.2mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal MDR1/ABCB1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Recognizes a discontinuous extracellular epitope on P-glycoprotein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: MDR1/ABCB1 Antibody (MRK16) [NBP2-52654] - Flow-cytometry using the MDR1/ABCB1 Antibody (MRK16) [NBP2-52654]. HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (NBP2-52654, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
