11-2439-41

antibody from Invitrogen Antibodies

Targeting: ABCB1 ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1

Antibody data

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Validation data

Product number: 11-2439-41 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD243 (ABCB1) Monoclonal Antibody (UIC2), FITC, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: This monoclonal antibody reacts with human Multidrug Resistant (MDR)-1, which is also known as P-glycoprotein (Pgp) and CD243. A 170-kDa transmembrane protein, MDR-1 is an ATP-dependent efflux pump for lipophilic compounds, including anti-cancer drugs. Expression of MDR-1 has been shown to correlate with multidrug resistance. In fact, tumor resistance to chemotherapy has been linked to MDR-1 expression in many cancers. MDR-1 is expressed in a variety of tissues, including the brain, kidney, liver, pancreas, and testes. Within the immune system, this molecule can be found on normal T, B, and natural killer cells, but not on monocytes.
Conjugate: Green dye
Antibody clone number: UIC2
Concentration: 5 µL/Test

ABCB1 protein structure - 11-2439-41 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Characterization of DCV-SP cells using pharmacological inhibition and antibody-based costaining of SP-conferring drug transporters. A2780V cells containing ABCB1-positive SP (a) and parental A2780 cells harbouring an ABCG2-positive SP (b) were stained with 10 mu M DCV (10 6 cells/mL) and subjected to a set of control experiments. Specifically, the samples were inhibited using 50 mu M verapamil (blocking several drug transporters) or 20 mu M fumitremorgin C (blocking ABCG2 specifically) or, where indicated, both. In addition, noninhibited and inhibited cells were costained for the drug transporters ABCB1 and ABCG2 using respective monoclonal antibodies. This multimodal approach helped us to better define particularly A2780V cells, whose SP contains a major population of ABCB1-positive cells (~8-10%) and a minor population of ABCG2-expressing cells (~0.1%). Note that, at least in our model systems, verapamil does not inhibit the activity of ABCG2.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 8 SP detection using the second-generation DyeCycle dyes DCG and DCO. A2780V cells harbouring an ABCB1-positive SP (a) and parental A2780 cells containing an ABCG2-positive SP (b) were stained with 500 nM of either DCG or DCO (10 6 cells/mL) and analysed by flow cytometry using the indicated emission filters (DCO data accentuated by dotted lines). For control purpose, verapamil (A2780V) or fumitremorgin C inhibition (A2780) was performed, and drug transporters were stained with APC-conjugated monoclonal antibodies. Both DCG and DCO are able to detect ABCB1-positive SP cells, whereas neither dye can identify SP cells that express ABCG2. Note the extreme separation of ABCB1-SP cells, which made it impossible to measure the fluorescence linearly.
Conjugate: Green dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1. Down-regulation of HOTAIR decreased the resistance of C666-1/DDP and CNE2/DDP cells to DDP. HOTAIR siRNA was transfected into C666-1/DDP and CNE2/DDP cells, following transfection for 48 h, the interference efficiencies were detected with qPCR (a). The IC 50 values of DDP (b, c), the protein levels of MDR1, MRP5, LRP1and ABCB1 (d, e) were detected by Western blotting. * p < 0.05, ** p < 0.01.
Conjugate: Green dye