11-2439-42
antibody from Invitrogen Antibodies
Targeting: ABCB1
ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 11-2439-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD243 (ABCB1) Monoclonal Antibody (UIC2), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This monoclonal antibody reacts with human Multidrug Resistant (MDR)-1, which is also known as P-glycoprotein (Pgp) and CD243. A 170-kDa transmembrane protein, MDR-1 is an ATP-dependent efflux pump for lipophilic compounds, including anti-cancer drugs. Expression of MDR-1 has been shown to correlate with multidrug resistance. In fact, tumor resistance to chemotherapy has been linked to MDR-1 expression in many cancers. MDR-1 is expressed in a variety of tissues, including the brain, kidney, liver, pancreas, and testes. Within the immune system, this molecule can be found on normal T, B, and natural killer cells, but not on monocytes. This antibody has been reported to inhibit MDR1-mediated efflux. Applications Reported: This UIC2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UIC2 antibody has been pre-titrated and tested by flow cytometric analysis on multidrug resistant cell lines. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- UIC2
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references HOTAIR mediates cisplatin resistance in nasopharyngeal carcinoma by regulating miR-106a-5p/SOX4 axis.
Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype.
Cao W, Sun Y, Liu L, Yu J, Ji J, Wang Y, Yang J
Bioengineered 2022 Mar;13(3):6567-6578
Bioengineered 2022 Mar;13(3):6567-6578
Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype.
Boesch M, Wolf D, Sopper S
Stem cells international 2016;2016:1652389
Stem cells international 2016;2016:1652389
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the 8226/S (left) and 8226 DOX40 (right) cell lines with Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (blue histogram) or Anti-Human CD243 (ABCB1) PE (purple histogram). Total viable cells were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Characterization of DCV-SP cells using pharmacological inhibition and antibody-based costaining of SP-conferring drug transporters. A2780V cells containing ABCB1-positive SP (a) and parental A2780 cells harbouring an ABCG2-positive SP (b) were stained with 10 mu M DCV (10 6 cells/mL) and subjected to a set of control experiments. Specifically, the samples were inhibited using 50 mu M verapamil (blocking several drug transporters) or 20 mu M fumitremorgin C (blocking ABCG2 specifically) or, where indicated, both. In addition, noninhibited and inhibited cells were costained for the drug transporters ABCB1 and ABCG2 using respective monoclonal antibodies. This multimodal approach helped us to better define particularly A2780V cells, whose SP contains a major population of ABCB1-positive cells (~8-10%) and a minor population of ABCG2-expressing cells (~0.1%). Note that, at least in our model systems, verapamil does not inhibit the activity of ABCG2.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 SP detection using the second-generation DyeCycle dyes DCG and DCO. A2780V cells harbouring an ABCB1-positive SP (a) and parental A2780 cells containing an ABCG2-positive SP (b) were stained with 500 nM of either DCG or DCO (10 6 cells/mL) and analysed by flow cytometry using the indicated emission filters (DCO data accentuated by dotted lines). For control purpose, verapamil (A2780V) or fumitremorgin C inhibition (A2780) was performed, and drug transporters were stained with APC-conjugated monoclonal antibodies. Both DCG and DCO are able to detect ABCB1-positive SP cells, whereas neither dye can identify SP cells that express ABCG2. Note the extreme separation of ABCB1-SP cells, which made it impossible to measure the fluorescence linearly.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Down-regulation of HOTAIR decreased the resistance of C666-1/DDP and CNE2/DDP cells to DDP. HOTAIR siRNA was transfected into C666-1/DDP and CNE2/DDP cells, following transfection for 48 h, the interference efficiencies were detected with qPCR (a). The IC 50 values of DDP (b, c), the protein levels of MDR1, MRP5, LRP1and ABCB1 (d, e) were detected by Western blotting. * p < 0.05, ** p < 0.01.
- Conjugate
- Green dye