17-2439-42
antibody from Invitrogen Antibodies
Targeting: ABCB1
ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 17-2439-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD243 (ABCB1) Monoclonal Antibody (UIC2), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This monoclonal antibody reacts with human Multidrug Resistant (MDR)-1, which is also known as P-glycoprotein (Pgp) and CD243. A 170-kDa transmembrane protein, MDR-1 is an ATP-dependent efflux pump for lipophilic compounds, including anti-cancer drugs. Expression of MDR-1 has been shown to correlate with multidrug resistance. In fact, tumor resistance to chemotherapy has been linked to MDR-1 expression in many cancers. MDR-1 is expressed in a variety of tissues, including the brain, kidney, liver, pancreas, and testes. Within the immune system, this molecule can be found on normal T, B, and natural killer cells, but not on monocytes. This antibody has been reported to inhibit MDR1-mediated efflux. Applications Reported: This UIC2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UIC2 antibody has been pre-titrated and tested This UIC2 antibody has been pre-titrated and tested by flow cytometric analysis on the multidrug resistant cell line 8226 DOX40 and its parent line 8226/S. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UIC2
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Targeting histone methylation to reprogram the transcriptional state that drives survival of drug-tolerant myeloid leukemia persisters.
Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype.
A double blinded, placebo-controlled pilot study to examine reduction of CD34 (+)/CD117 (+)/CD133 (+) lymphoma progenitor cells and duration of remission induced by neoadjuvant valspodar in dogs with large B-cell lymphoma.
Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody.
Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes.
van Gils N, Verhagen HJMP, Broux M, Martiáñez T, Denkers F, Vermue E, Rutten A, Csikós T, Demeyer S, Çil M, Al M, Cools J, Janssen JJWM, Ossenkoppele GJ, Menezes RX, Smit L
iScience 2022 Sep 16;25(9):105013
iScience 2022 Sep 16;25(9):105013
Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype.
Boesch M, Wolf D, Sopper S
Stem cells international 2016;2016:1652389
Stem cells international 2016;2016:1652389
A double blinded, placebo-controlled pilot study to examine reduction of CD34 (+)/CD117 (+)/CD133 (+) lymphoma progenitor cells and duration of remission induced by neoadjuvant valspodar in dogs with large B-cell lymphoma.
Ito D, Childress M, Mason N, Winter A, O'Brien T, Henson M, Borgatti A, Lewellen M, Krick E, Stewart J, Lahrman S, Rajwa B, Scott MC, Seelig D, Koopmeiners J, Ruetz S, Modiano J
F1000Research 2015;4:42
F1000Research 2015;4:42
Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody.
Mechetner EB, Roninson IB
Proceedings of the National Academy of Sciences of the United States of America 1992 Jul 1;89(13):5824-8
Proceedings of the National Academy of Sciences of the United States of America 1992 Jul 1;89(13):5824-8
Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes.
Chaudhary PM, Mechetner EB, Roninson IB
Blood 1992 Dec 1;80(11):2735-9
Blood 1992 Dec 1;80(11):2735-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the 8226/S (left) and 8226 DOX40 (right) cell lines with Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (blue histogram) or Anti-Human CD243 (ABCB1) APC (purple histogram). Total viable cells were used for analysis.