A00049-1
antibody from Boster Biological Technology
Targeting: ABCB1
ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1
Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A00049-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-P Glycoprotein/ABCB1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for P Glycoprotein detection. Tested with WB, ICC/IF, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Gastric cancer mesenchymal stem cells promote tumor glycolysis and chemoresistance by regulating B7H3 in gastric cancer cells.
Photoactivated DNA Nanodrugs Damage Mitochondria to Improve Gene Therapy for Reversing Chemoresistance.
Evaluation and antitumor mechanism of functionalized chitosan-based polymeric micelles for oral delivery of paclitaxel.
A Novel Drug Modulator Diarylheptanoid (trans-1,7-Diphenyl-5-hydroxy-1-heptene) from Curcuma comosa Rhizomes for P-glycoprotein Function and Apoptosis Induction in K652/ADR Leukemic Cells.
P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3.
A 20(S)-protopanoxadiol derivative overcomes multi-drug resistance by antagonizing ATP-binding cassette subfamily B member 1 transporter function.
Shugoshin1 enhances multidrug resistance of gastric cancer cells by regulating MRP1, Bcl-2, and Bax genes.
Expression and significance of hypoxia-inducible factor-1 alpha and MDR1/P-glycoprotein in human colon carcinoma tissue and cells.
Growth retardation of fetal rats exposed to nicotine in utero: possible involvement of CYP1A1, CYP2E1, and P-glycoprotein.
Regulation of multidrug resistance by MGr1-antigen in gastric cancer cells.
Indole-3-carbinol alters placental cytochrome P450 1A1 and P-glycoprotein levels in rats: a potential role in intensifying fetal intrauterine growth-retardation produced by tobacco smoke.
Ribosomal proteins S13 and L23 promote multidrug resistance in gastric cancer cells by suppressing drug-induced apoptosis.
Overexpression of ZNRD1 promotes multidrug-resistant phenotype of gastric cancer cells through upregulation of P-glycoprotein.
Gao Q, Huang C, Liu T, Yang F, Chen Z, Sun L, Zhao Y, Wang M, Luo L, Zhou C, Zhu W
Journal of cellular biochemistry 2024 Mar;125(3):e30521
Journal of cellular biochemistry 2024 Mar;125(3):e30521
Photoactivated DNA Nanodrugs Damage Mitochondria to Improve Gene Therapy for Reversing Chemoresistance.
Wang D, Yi H, Geng S, Jiang C, Liu J, Duan J, Zhang Z, Shi J, Song H, Guo Z, Zhang K
ACS nano 2023 Sep 12;17(17):16923-16934
ACS nano 2023 Sep 12;17(17):16923-16934
Evaluation and antitumor mechanism of functionalized chitosan-based polymeric micelles for oral delivery of paclitaxel.
Wang X, Zheng Y, Qiu L, Ouyang H, Xu X, Xu W, Zhang Y, Xu W
International journal of pharmaceutics 2022 Sep 25;625:122138
International journal of pharmaceutics 2022 Sep 25;625:122138
A Novel Drug Modulator Diarylheptanoid (trans-1,7-Diphenyl-5-hydroxy-1-heptene) from Curcuma comosa Rhizomes for P-glycoprotein Function and Apoptosis Induction in K652/ADR Leukemic Cells.
Viriyaadhammaa N, Duangmano S, Saiai A, Tungjai M, Dejkriengkraikul P, Tima S, Chiampanichayakul S, Krise J, Anuchapreeda S
International journal of molecular sciences 2022 Aug 12;23(16)
International journal of molecular sciences 2022 Aug 12;23(16)
P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3.
Deng L, Li Q, Lin G, Huang D, Zeng X, Wang X, Li P, Jin X, Zhang H, Li C, Chen L, Wang L, Huang S, Shao H, Xu B, Mao J
Theranostics 2016;6(2):204-18
Theranostics 2016;6(2):204-18
A 20(S)-protopanoxadiol derivative overcomes multi-drug resistance by antagonizing ATP-binding cassette subfamily B member 1 transporter function.
Chen G, Liu J, Chen W, Xu Q, Xiao M, Hu L, Mao L, Wang X
Oncotarget 2016 Feb 23;7(8):9388-403
Oncotarget 2016 Feb 23;7(8):9388-403
Shugoshin1 enhances multidrug resistance of gastric cancer cells by regulating MRP1, Bcl-2, and Bax genes.
Wang Y, Liu L, Liu X, Zhang H, Liu J, Feng B, Shang Y, Zhou L, Wu K, Nie Y, Zhang H, Fan D
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2013 Aug;34(4):2205-14
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2013 Aug;34(4):2205-14
Expression and significance of hypoxia-inducible factor-1 alpha and MDR1/P-glycoprotein in human colon carcinoma tissue and cells.
Ding Z, Yang L, Xie X, Xie F, Pan F, Li J, He J, Liang H
Journal of cancer research and clinical oncology 2010 Nov;136(11):1697-707
Journal of cancer research and clinical oncology 2010 Nov;136(11):1697-707
Growth retardation of fetal rats exposed to nicotine in utero: possible involvement of CYP1A1, CYP2E1, and P-glycoprotein.
Wang T, Chen M, Yan YE, Xiao FQ, Pan XL, Wang H
Environmental toxicology 2009 Feb;24(1):33-42
Environmental toxicology 2009 Feb;24(1):33-42
Regulation of multidrug resistance by MGr1-antigen in gastric cancer cells.
Sun L, Shi Y, Guo C, Yao L, Lin T, Du J, Han Q, Han Y, Fan D
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2006;27(1):27-35
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2006;27(1):27-35
Indole-3-carbinol alters placental cytochrome P450 1A1 and P-glycoprotein levels in rats: a potential role in intensifying fetal intrauterine growth-retardation produced by tobacco smoke.
Yan YE, Wang H, Wang T, Zeng HG
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2006 Aug;58(1):39-47
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2006 Aug;58(1):39-47
Ribosomal proteins S13 and L23 promote multidrug resistance in gastric cancer cells by suppressing drug-induced apoptosis.
Shi Y, Zhai H, Wang X, Han Z, Liu C, Lan M, Du J, Guo C, Zhang Y, Wu K, Fan D
Experimental cell research 2004 Jun 10;296(2):337-46
Experimental cell research 2004 Jun 10;296(2):337-46
Overexpression of ZNRD1 promotes multidrug-resistant phenotype of gastric cancer cells through upregulation of P-glycoprotein.
Shi Y, Zhang Y, Zhao Y, Hong L, Liu N, Jin X, Pan Y, Fan D
Cancer biology & therapy 2004 Apr;3(4):377-81
Cancer biology & therapy 2004 Apr;3(4):377-81
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of P Glycoprotein using anti-P Glycoprotein antibody (A00049-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates,Lane 2: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P Glycoprotein antigen affinity purified polyclonal antibody (Catalog # A00049-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P Glycoprotein at approximately 160KD. The expected band size for P Glycoprotein is at 141KD.
- Additional image