LS-C745304

antibody from LSBio

Targeting: ABCB1 ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C745304

Provider

LSBio

Product name

ABCB1 / MDR1 / P Glycoprotein Antibody LS-C745304

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Canine, Simian

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using the affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10,000.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC using ABCB1 Antibody at 20 ug/ml against FFPE human kidney. Moderate to strong cytoplasmic and membranous staining seen in this example. Similar staining also observed in other tissues including liver, kidney, and small intestine. The antibody showed minimal background staining and worked well in formalin-fixed tissues.