A00049-3

antibody from Boster Biological Technology

Targeting: ABCB1 ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1

Provider product page for A00049-3

Western blot

ELISA

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [14]
Comments [0]
Validations
Western blot [1]

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Product number: A00049-3 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-P Glycoprotein/ABCB1 Antibody Picoband™
Antibody type: Polyclonal
Description: Rabbit IgG polyclonal antibody for P Glycoprotein/ABCB1 detection. Tested with WB, IHC-P, FCM, Direct ELISA in Human;Mouse;Rat.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Vial size: 100μg/vial
Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Handling: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ABCB1 protein structure - A00049-3 shown in red.

Submitted references Gastric cancer mesenchymal stem cells promote tumor glycolysis and chemoresistance by regulating B7H3 in gastric cancer cells.

Gao Q, Huang C, Liu T, Yang F, Chen Z, Sun L, Zhao Y, Wang M, Luo L, Zhou C, Zhu W
Journal of cellular biochemistry 2024 Mar;125(3):e30521

Evaluation and antitumor mechanism of functionalized chitosan-based polymeric micelles for oral delivery of paclitaxel.

Wang X, Zheng Y, Qiu L, Ouyang H, Xu X, Xu W, Zhang Y, Xu W
International journal of pharmaceutics 2022 Sep 25;625:122138

A Novel Drug Modulator Diarylheptanoid (trans-1,7-Diphenyl-5-hydroxy-1-heptene) from Curcuma comosa Rhizomes for P-glycoprotein Function and Apoptosis Induction in K652/ADR Leukemic Cells.

Viriyaadhammaa N, Duangmano S, Saiai A, Tungjai M, Dejkriengkraikul P, Tima S, Chiampanichayakul S, Krise J, Anuchapreeda S
International journal of molecular sciences 2022 Aug 12;23(16)

A 20(S)-protopanoxadiol derivative overcomes multi-drug resistance by antagonizing ATP-binding cassette subfamily B member 1 transporter function.

Chen G, Liu J, Chen W, Xu Q, Xiao M, Hu L, Mao L, Wang X
Oncotarget 2016 Feb 23;7(8):9388-403

Regulation of P-glycoprotein efflux activity by Z-guggulsterone of Commiphora mukul at the blood-brain barrier.

Xu HB, Yu J, Xu LZ, Fu J
Journal of the neurological sciences 2016 Apr 15;363:147-52

Alteration in P-glycoprotein at the blood-brain barrier in the early period of MCAO in rats.

Cen J, Liu L, Li MS, He L, Wang LJ, Liu YQ, Liu M, Ji BS
The Journal of pharmacy and pharmacology 2013 May;65(5):665-72

In vitro and in vivo study of dolichyl phosphate on the efflux activity of P-glycoprotein at the blood-brain barrier.

Ji BS, Cen J, Liu L, He L
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 2013 Dec;31(8):828-35

Shugoshin1 enhances multidrug resistance of gastric cancer cells by regulating MRP1, Bcl-2, and Bax genes.

Wang Y, Liu L, Liu X, Zhang H, Liu J, Feng B, Shang Y, Zhou L, Wu K, Nie Y, Zhang H, Fan D
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2013 Aug;34(4):2205-14

Reversion of multidrug resistance by co-encapsulation of doxorubicin and curcumin in chitosan/poly(butyl cyanoacrylate) nanoparticles.

Duan J, Mansour HM, Zhang Y, Deng X, Chen Y, Wang J, Pan Y, Zhao J
International journal of pharmaceutics 2012 Apr 15;426(1-2):193-201

Various doses of fractioned irradiation modulates multidrug resistance 1 expression differently through hypoxia-inducible factor 1α in esophageal cancer cells.

Zhang GJ, Gao R, Wang JS, Fu JK, Zhang MX, Jin X
Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus 2011 Sep;24(7):481-8

Design and implementation of electrochemical cytosensor for evaluation of cell surface carbohydrate and glycoprotein.

Zhang JJ, Cheng FF, Zheng TT, Zhu JJ
Analytical chemistry 2010 May 1;82(9):3547-55

Glutathione depletion upregulates P-glycoprotein expression at the blood-brain barrier in rats.

Wu J, Ji H, Wang YY, Wang Y, Li YQ, Li WG, Long Y, Xia YZ, Hong H
The Journal of pharmacy and pharmacology 2009 Jun;61(6):819-24

Regulation of multidrug resistance by MGr1-antigen in gastric cancer cells.

Sun L, Shi Y, Guo C, Yao L, Lin T, Du J, Han Q, Han Y, Fan D
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2006;27(1):27-35

Indole-3-carbinol alters placental cytochrome P450 1A1 and P-glycoprotein levels in rats: a potential role in intensifying fetal intrauterine growth-retardation produced by tobacco smoke.

Yan YE, Wang H, Wang T, Zeng HG
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2006 Aug;58(1):39-47

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Supportive validation

Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: Western blot analysis of P Glycoprotein/ABCB1 using anti-P Glycoprotein/ABCB1 antibody (A00049-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P Glycoprotein/ABCB1 antigen affinity purified polyclonal antibody (Catalog # A00049-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P Glycoprotein/ABCB1 at approximately 140KD. The expected band size for P Glycoprotein/ABCB1 is at 140KD.
Additional image