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LearnPA5-31840
antibody from Invitrogen Antibodies
Targeting: SMG7
C1orf16, EST1C, KIAA0250, SGA56M, SMG-7
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-31840 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMG7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1. Predicted reactivity: Human (99%), Mouse (94%), Rat (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMG7 using 30 µg of NT2D1 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a SMG7 polyclonal antibody (Product # PA5-31840) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SMG7 Polyclonal Antibody (Product # PA5-31840). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMG7 Polyclonal Antibody (Product # PA5-31840) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SMG7 Polyclonal Antibody (Product # PA5-31840). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMG7 Polyclonal Antibody (Product # PA5-31840) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMG7 Polyclonal Antibody detects SMG7 protein at cytoplasm and nucleus in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. SMG7 Polyclonal Antibody (Product # PA5-31840) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
