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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- GTX54520 - Provider product page
- Provider
- GeneTex
- Product name
- Huntingtin (neoepitope 513) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Affinity chromatography
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- -20° C
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of endogenous Htt lysates with or without different caspase activity (lanes 1-5) and overexpressed recombinant Htt fragment lysates (lanes 6-9) (20 ug per well) using pan-Huntingtin antibody (top, N-terminal HTT) or Huntingtin (neoepitope 513) antibody at a dilution of 1:500 (bottom, neoHtt513).
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sandwich ELISA was performed with a Huntingtin monoclonal antibody and Huntingtin (neoepitope 513) antibody to determine the antigen concentration of the Huntingtin cleavage products. The curve represents a dose response for neo513 in 293T cells overexpressing the Htt construct.
- Validation comment
- ELISA
