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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX54522 - Provider product page
- Provider
- GeneTex
- Product name
- Huntingtin (neoepitope 586) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of endogenous Htt lysates with or without different caspase activity (lanes 1-5) and overexpressed recombinant Htt fragment lysates (lanes 6-9) (20 ug per well) using pan-Huntingtin antibody (top, N-terminal HTT) or Huntingtin (neoepitope 586) antibody at a dilution of 1:500 (bottom, neoHtt586).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sandwich ELISA was performed with a Huntingtin monoclonal antibody and Huntingtin (neoepitope 586) antibody to determine the antigen concentration of the Huntingtin cleavage products. The curve represents a dose response for neo586 in 293T cells overexpressing the Htt construct.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of Caspase cleaved Htt (green) in 293T cells transfected with Htt23Q and Htt148Q stop constructs ending in amino acid 586 using Huntingtin (neoepitope 586) antibody at a dilution of 1:50 (green). Nuclei (blue) were stained with Hoechst dye.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC analysis of tissues from either transgenic HD mice of the YAC128 line (left) or their WT littermates (right). Striatum (top panels) and cortex (bottom panels) tissues were stained using Huntingtin (neoepitope 586) antibody at a dilution of 1:50 (green).
