H00003064-M05

antibody from Abnova Corporation

Targeting: HTT HD, IT15

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003064-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003064-M05, RRID:AB_606367

Product name

HD monoclonal antibody (M05), clone 1H6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant HD.

Antigen sequence

AVAEEPLHRPKKELSATKKDRVNHCLTICENIVAQ
SVRNSPEFQKLLGIAMELFLLCSDDAESDVRMVAD
ECLNKVIKALMDSNLPRLQLELYKEIKKNGAPRSL
RAALW


Isotype

IgG

Antibody clone number

1H6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

IKK phosphorylates Huntingtin and targets it for degradation by the proteasome and lysosome.

Thompson LM, Aiken CT, Kaltenbach LS, Agrawal N, Illes K, Khoshnan A, Martinez-Vincente M, Arrasate M, O'Rourke JG, Khashwji H, Lukacsovich T, Zhu YZ, Lau AL, Massey A, Hayden MR, Zeitlin SO, Finkbeiner S, Green KN, LaFerla FM, Bates G, Huang L, Patterson PH, Lo DC, Cuervo AM, Marsh JL, Steffan JS
The Journal of cell biology 2009 Dec 28;187(7):1083-99

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HD is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol