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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-26250 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fibromodulin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The small leucine rich proteoglycan fibromodulin is overexpressed in human prostate epithelial cancer cell lines in culture and human prostate cancer tissue.
Reyes N, Benedetti I, Bettin A, Rebollo J, Geliebter J
Cancer biomarkers : section A of Disease markers 2016;16(1):191-202
Cancer biomarkers : section A of Disease markers 2016;16(1):191-202
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Fibromodulin in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with Fibromodulin polyclonal antibody (Product # PA5-26250) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HeLa cells using a FMOD polyclonal antibody (Product # PA5-26250) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Fibromodulin in Hela cells (right histogram). Samples were incubated with Fibromodulin polyclonal antibody (Product # PA5-26250) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
