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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3207 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3207, RRID:AB_1578754
- Product name
- PIK3C2B polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PIK3C2B.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Human phosphoinositide 3-kinase C2beta, the role of calcium and the C2 domain in enzyme activity.
Identification and cDNA cloning of a novel mammalian C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K.
Arcaro A, Volinia S, Zvelebil MJ, Stein R, Watton SJ, Layton MJ, Gout I, Ahmadi K, Downward J, Waterfield MD
The Journal of biological chemistry 1998 Dec 4;273(49):33082-90
The Journal of biological chemistry 1998 Dec 4;273(49):33082-90
Identification and cDNA cloning of a novel mammalian C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K.
Brown RA, Ho LK, Weber-Hall SJ, Shipley JM, Fry MJ
Biochemical and biophysical research communications 1997 Apr 17;233(2):537-44
Biochemical and biophysical research communications 1997 Apr 17;233(2):537-44
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3C2B polyclonal antibody (Cat # PAB3207) in HL-60 cell lysate. PIK3C2B (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Validation comment
- Western Blot (Cell lysate)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PIK3C2B polyclonal antibody (Cat # PAB3207) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
