NB600-642-100ug

antibody from Novus Biologicals

Targeting: TYMS HsT422, TMS, TS, Tsase

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NB600-642-100ug

Provider

Novus Biologicals

Product name

Sheep Polyclonal Thymidylate Synthase Antibody

Antibody type

Polyclonal

Description

IgG purified. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. The antibody recognizes the 36,000 MW hTS. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required for some non-human cell lines. Anti-Thymidylate Synthase can detect TS by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining, however, some colorectal cancer specimens show very strong staining. Reactivity in other immunoassays is unknown.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Thymidylate Synthase Antibody [NB600-642] - Detection thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB substrate for approximately 4'.