Antibody data
- Antibody Data
- Antigen structure
- References [28]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 15047-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15047-1-AP, RRID:AB_2210721
- Product name
- Thymidylate synthase antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Thymidylate synthase antibody (Cat. #15047-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Thymidylate synthase disruption to limit cell proliferation in cell therapies.
Thymidylate synthase promotes esophageal squamous cell carcinoma growth by relieving oxidative stress through activating nuclear factor erythroid 2-related factor 2 expression.
Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions.
Pemetrexed induces ROS generation and cellular senescence by attenuating TS-mediated thymidylate metabolism to reverse gefitinib resistance in NSCLC.
Lysine butyrylation of HSP90 regulated by KAT8 and HDAC11 confers chemoresistance.
Therapeutic potential of Clostridium butyricum anticancer effects in colorectal cancer.
lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.
A Prognostic Model of Differentiated Thyroid Cancer Based on Up-Regulated Glycolysis-Related Genes.
Comprehensive serum metabolomics and network analysis to reveal the mechanism of gypenosides in treating lung cancer and enhancing the pharmacological effects of cisplatin.
The Implication of Serum Autoantibodies in Prognosis of Canine Mammary Tumors.
CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.
A Novel Integrated Metabolism-Immunity Gene Expression Model Predicts the Prognosis of Lung Adenocarcinoma Patients.
Identification and Validation of TYMS as a Potential Biomarker for Risk of Metastasis Development in Hepatocellular Carcinoma.
Significant association of serum autoantibodies to TYMS, HAPLN1 and IGFBP5 with early stage canine malignant mammary tumours.
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Uncovering the anti-NSCLC effects and mechanisms of gypenosides by metabolomics and network pharmacology analysis.
5-Fluorouracil Enhances the Antitumor Activity of the Glutaminase Inhibitor CB-839 against PIK3CA-Mutant Colorectal Cancers.
MicroRNA-375-3p enhances chemosensitivity to 5-fluorouracil by targeting thymidylate synthase in colorectal cancer.
Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.
Pemetrexed inhibits Kaposi's sarcoma-associated herpesvirus replication through blocking dTMP synthesis.
Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.
MEK inhibitor enhanced the antitumor effect of oxaliplatin and 5‑fluorouracil in MEK1 Q56P‑mutant colorectal cancer cells.
An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactions.
The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.
Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells.
CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.
Suberoylanilide hydroxamic acid enhances chemosensitivity to 5-fluorouracil in hepatocellular carcinoma via inhibition of thymidylate synthase.
Screening for Target Engagement using the Cellular Thermal Shift Assay - CETSA.
Sartori-Maldonado R, Montaser H, Soppa I, Eurola S, Juutila J, Balaz M, Puttonen H, Otonkoski T, Saarimäki-Vire J, Wartiovaara K
Molecular therapy : the journal of the American Society of Gene Therapy 2024 Aug 7;32(8):2535-2548
Molecular therapy : the journal of the American Society of Gene Therapy 2024 Aug 7;32(8):2535-2548
Thymidylate synthase promotes esophageal squamous cell carcinoma growth by relieving oxidative stress through activating nuclear factor erythroid 2-related factor 2 expression.
Yang J, Zhang J, Chen J, Yang X, Sun H, Zhao Z, Zhou H, Shen H
PloS one 2023;18(9):e0290264
PloS one 2023;18(9):e0290264
Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions.
Skinner OS, Blanco-Fernández J, Goodman RP, Kawakami A, Shen H, Kemény LV, Joesch-Cohen L, Rees MG, Roth JA, Fisher DE, Mootha VK, Jourdain AA
Nature metabolism 2023 May;5(5):765-776
Nature metabolism 2023 May;5(5):765-776
Pemetrexed induces ROS generation and cellular senescence by attenuating TS-mediated thymidylate metabolism to reverse gefitinib resistance in NSCLC.
Chen Y, Zhang C, Jin S, Li J, Dai J, Zhang Z, Guo R
Journal of cellular and molecular medicine 2023 Jul;27(14):2032-2044
Journal of cellular and molecular medicine 2023 Jul;27(14):2032-2044
Lysine butyrylation of HSP90 regulated by KAT8 and HDAC11 confers chemoresistance.
He Y, Zheng CC, Yang J, Li SJ, Xu TY, Wei X, Chen WY, Jiang ZL, Xu JJ, Zhang GG, Cheng C, Chen KS, Shi XY, Qin DJ, Liu JB, Li B
Cell discovery 2023 Jul 18;9(1):74
Cell discovery 2023 Jul 18;9(1):74
Therapeutic potential of Clostridium butyricum anticancer effects in colorectal cancer.
Xu H, Luo H, Zhang J, Li K, Lee MH
Gut microbes 2023 Jan-Dec;15(1):2186114
Gut microbes 2023 Jan-Dec;15(1):2186114
lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.
Liang G, Zeng M, Gao M, Xing W, Jin X, Wang Q, Deng L, Ou H, He Z
Oxidative medicine and cellular longevity 2022;2022:9369035
Oxidative medicine and cellular longevity 2022;2022:9369035
A Prognostic Model of Differentiated Thyroid Cancer Based on Up-Regulated Glycolysis-Related Genes.
Wu M, Ou-Yang DJ, Wei B, Chen P, Shi QM, Tan HL, Huang BQ, Liu M, Qin ZE, Li N, Hu HY, Huang P, Chang S
Frontiers in endocrinology 2022;13:775278
Frontiers in endocrinology 2022;13:775278
Comprehensive serum metabolomics and network analysis to reveal the mechanism of gypenosides in treating lung cancer and enhancing the pharmacological effects of cisplatin.
Qi YS, Xiao MY, Xie P, Xie JB, Guo M, Li FF, Piao XL
Frontiers in pharmacology 2022;13:1070948
Frontiers in pharmacology 2022;13:1070948
The Implication of Serum Autoantibodies in Prognosis of Canine Mammary Tumors.
Yuan SH, Chang SC, Chou PY, Yang Y, Liu HP
Animals : an open access journal from MDPI 2022 Sep 18;12(18)
Animals : an open access journal from MDPI 2022 Sep 18;12(18)
CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.
Liang YY, Bacanu S, Sreekumar L, Ramos AD, Dai L, Michaelis M, Cinatl J, Seki T, Cao Y, Coffill CR, Lane DP, Prabhu N, Nordlund P
Cell chemical biology 2022 Apr 21;29(4):572-585.e8
Cell chemical biology 2022 Apr 21;29(4):572-585.e8
A Novel Integrated Metabolism-Immunity Gene Expression Model Predicts the Prognosis of Lung Adenocarcinoma Patients.
Chen S, Duan Y, Wu Y, Yang D, An J
Frontiers in pharmacology 2021;12:728368
Frontiers in pharmacology 2021;12:728368
Identification and Validation of TYMS as a Potential Biomarker for Risk of Metastasis Development in Hepatocellular Carcinoma.
Li S, Zhao J, Lv L, Dong D
Frontiers in oncology 2021;11:762821
Frontiers in oncology 2021;11:762821
Significant association of serum autoantibodies to TYMS, HAPLN1 and IGFBP5 with early stage canine malignant mammary tumours.
Chang SC, Yuan SH, Li CY, Chang HM, Wang HC, Pan YA, Hsueh PC, Wu CC, Yang Y, Liu HP
Veterinary and comparative oncology 2021 Mar;19(1):172-182
Veterinary and comparative oncology 2021 Mar;19(1):172-182
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Cui L, Zhao X, Jin Z, Wang H, Yang SF, Hu S
Journal of pineal research 2021 Dec;71(4):e12767
Journal of pineal research 2021 Dec;71(4):e12767
Uncovering the anti-NSCLC effects and mechanisms of gypenosides by metabolomics and network pharmacology analysis.
Qi YS, Xie JB, Xie P, Duan Y, Ling YQ, Gu YL, Piao XL
Journal of ethnopharmacology 2021 Dec 5;281:114506
Journal of ethnopharmacology 2021 Dec 5;281:114506
5-Fluorouracil Enhances the Antitumor Activity of the Glutaminase Inhibitor CB-839 against PIK3CA-Mutant Colorectal Cancers.
Zhao Y, Feng X, Chen Y, Selfridge JE, Gorityala S, Du Z, Wang JM, Hao Y, Cioffi G, Conlon RA, Barnholtz-Sloan JS, Saltzman J, Krishnamurthi SS, Vinayak S, Veigl M, Xu Y, Bajor DL, Markowitz SD, Meropol NJ, Eads JR, Wang Z
Cancer research 2020 Nov 1;80(21):4815-4827
Cancer research 2020 Nov 1;80(21):4815-4827
MicroRNA-375-3p enhances chemosensitivity to 5-fluorouracil by targeting thymidylate synthase in colorectal cancer.
Xu F, Ye ML, Zhang YP, Li WJ, Li MT, Wang HZ, Qiu X, Xu Y, Yin JW, Hu Q, Wei WH, Chang Y, Liu L, Zhao Q
Cancer science 2020 May;111(5):1528-1541
Cancer science 2020 May;111(5):1528-1541
Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.
Fu F, Zhang Y, Gao Z, Zhao Y, Wen Z, Han H, Li Y, Chen H
International journal of cancer 2020 Jul 15;147(2):584-592
International journal of cancer 2020 Jul 15;147(2):584-592
Pemetrexed inhibits Kaposi's sarcoma-associated herpesvirus replication through blocking dTMP synthesis.
Chen J, Zhang H, Chen X
Antiviral research 2020 Aug;180:104825
Antiviral research 2020 Aug;180:104825
Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.
Xie W, Wang B, Wang X, Hou D, Su H, Huang H
Annals of translational medicine 2020 Apr;8(7):478
Annals of translational medicine 2020 Apr;8(7):478
MEK inhibitor enhanced the antitumor effect of oxaliplatin and 5‑fluorouracil in MEK1 Q56P‑mutant colorectal cancer cells.
Jing C, Li H, Du Y, Cao H, Liu S, Wang Z, Ma R, Feng J, Wu J
Molecular medicine reports 2019 Feb;19(2):1092-1100
Molecular medicine reports 2019 Feb;19(2):1092-1100
An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactions.
Lim YT, Prabhu N, Dai L, Go KD, Chen D, Sreekumar L, Egeblad L, Eriksson S, Chen L, Veerappan S, Teo HL, Tan CSH, Lengqvist J, Larsson A, Sobota RM, Nordlund P
PloS one 2018;13(12):e0208273
PloS one 2018;13(12):e0208273
The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.
Hoxhaj G, Hughes-Hallett J, Timson RC, Ilagan E, Yuan M, Asara JM, Ben-Sahra I, Manning BD
Cell reports 2017 Oct 31;21(5):1331-1346
Cell reports 2017 Oct 31;21(5):1331-1346
Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells.
Liang X, Shi H, Yang L, Qiu C, Lin S, Qi Y, Li J, Zhao A, Liu J
British journal of cancer 2017 Mar 28;116(7):903-911
British journal of cancer 2017 Mar 28;116(7):903-911
CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.
Almqvist H, Axelsson H, Jafari R, Dan C, Mateus A, Haraldsson M, Larsson A, Martinez Molina D, Artursson P, Lundbäck T, Nordlund P
Nature communications 2016 Mar 24;7:11040
Nature communications 2016 Mar 24;7:11040
Suberoylanilide hydroxamic acid enhances chemosensitivity to 5-fluorouracil in hepatocellular carcinoma via inhibition of thymidylate synthase.
Liao B, Liang H, Chen J, Liu Q, Zhang B, Chen X
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Dec;36(12):9347-56
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Dec;36(12):9347-56
Screening for Target Engagement using the Cellular Thermal Shift Assay - CETSA.
Markossian S, Grossman A, Arkin M, Auld D, Austin C, Baell J, Brimacombe K, Chung TDY, Coussens NP, Dahlin JL, Devanarayan V, Foley TL, Glicksman M, Gorshkov K, Haas JV, Hall MD, Hoare S, Inglese J, Iversen PW, Lal-Nag M, Li Z, Manro JR, McGee J, McManus O, Pearson M, Riss T, Saradjian P, Sittampalam GS, Tarselli M, Trask OJ Jr, Weidner JR, Wildey MJ, Wilson K, Xia M, Xu X, Axelsson H, Almqvist H, Seashore-Ludlow B, Lundbäck T
2004;
2004;
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15047-1-AP(TYMS antibody) at dilution of 1:1500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using TYMS antibody 15047-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human endometrial cancer using 15047-1-AP(TYMS antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human endometrial cancer using 15047-1-AP(TYMS antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue