15047-1-AP

antibody from Invitrogen Antibodies

Targeting: TYMS HsT422, TMS, TS, Tsase

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15047-1-AP

Provider

Invitrogen Antibodies

Product name

Thymidylate synthase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPVAGSELP RRPLPPAAQE RDAEPRPPHG ELQYLGQIQH ILRCGVRKDD RTGTGTLSVF GMQARYSLRD EFPLLTTKRV FWKGVLEELL WFIKGSTNAK ELSSKGVKIW DANGSRDFLD SLGFSTREEG DLGPVYGFQW RHFGAEYRDM ESDYSGQGVD QLQRVIDTIK TNPDDRRIIM CAWNPRDLPL MALPPCHALC QFYVVNSELS CQLYQRSGDM GLGVPFNIAS YALLTYMIAH ITGLKPGDFI HTLGDAHIYL NHIEPLKIQL QREPRPFPKL RILRKVEKID DFKAEDFQIE GYNPHPTIKM EMAV (1-313 aa encoded by BC002567)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.31 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293T cells were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using TYMS antibody 15047-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human Thymidylate synthase (Product # 15047-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Thymidylate synthase (IP:15047-1-AP, 3ug; Detection:15047-1-AP 1:600) with HeLa cells lysate 3200ug.