Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
- Other assay [2]
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Validation data
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- Product number
- 7298-MSM4-P0 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thymidylate Synthase Monoclonal Antibody (TYMS, 1884)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- TYMS, 1884
- Vial size
- 20 µg
- Concentration
- 200 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of human HeLa cell lysate using Thymidylate Synthase Monoclonal Antibody (Product # 7298-MSM4-P1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of PFA-fixed Ramos cells using Thymidylate Synthase Monoclonal Antibody (Product # 7298-MSM4-P1) followed by goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human tonsil using Thymidylate Synthase Monoclonal Antibody (Product # 7298-MSM4-P1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of PFA-fixed MOLT4 cells using Thymidylate Synthase Monoclonal Antibody (Product # 7298-MSM4-P1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of PFA-fixed Ramos cells using Thymidylate Synthase Monoclonal Antibody (Product # 7298-MSM4-P1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Analysis of Protein Array containing more than 19,000 full-length human proteins Thymidylate Synthase Monoclonal Antibody (Product # 7298-MSM4-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDís) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDís) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDS-PAGE Analyis Purified Thymidylate Synthase Monoclonal Antibody (Product # 7298-MSM4-P1). Confirmation of Purity and Integrity of Antibody.