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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-22263 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thymidylate Synthase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116. Predicted reactivity: Mouse (94%), Rat (95%), Zebrafish (88%), Xenopus laevis (85%), Dog (96%), Pig (96%), Rhesus Monkey (98%), Chimpanzee (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TS using 30 µg of H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TS polyclonal antibody (Product # PA5-22263) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Thymidylate Synthase was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Thymidylate Synthase Polyclonal Antibody (Product # PA5-22263) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Thymidylate Synthase Polyclonal Antibody (Product # PA5-22263). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Thymidylate synthetase antibody [N3C3] Thymidylate Synthase Polyclonal Antibody (Product # PA5-22263) diluted at 1:1,000.
