Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-27867 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thymidylate Synthase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Jurkat, Raji, THP-1, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thymidylate synthetase antibody detects Thymidylate synthetase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthetase antibody Thymidylate Synthase Polyclonal Antibody (Product # PA5-27867) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Thymidylate Synthase Polyclonal Antibody (Product # PA5-27867). Sample (30 µg of whole cell lysate). Lane A: Jurkat. Lane B: Raji. Lane C: THP-1. 10% SDS PAGE. Thymidylate Synthase Polyclonal Antibody (Product # PA5-27867) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thymidylate synthetase antibody detects Thymidylate synthetase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthetase antibody Thymidylate Synthase Polyclonal Antibody (Product # PA5-27867) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thymidylate synthetase antibody detects TYMS protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract. F. 30 µg Raw264.7 whole cell lysate/extract. G. 30 µg C2C12 whole cell lysate/extract.10 % SDS-PAGE. Thymidylate synthetase antibody Thymidylate Synthase Polyclonal Antibody (Product # PA5-27867) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thymidylate synthetase antibody detects TYMS protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.10 % SDS-PAGE. Thymidylate synthetase antibody Thymidylate Synthase Polyclonal Antibody (Product # PA5-27867) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Thymidylate synthetase showing staining in the cytoplasm of A375 cells. A375 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using a Thymidylate synthetase polyclonal antibody (Product # PA5-27867) diluted at 1:1000. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thymidylate Synthase Polyclonal Antibody detects Thymidylate synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thymidylate synthase stained by Thymidylate Synthase Polyclonal Antibody (Product # PA5-27867) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thymidylate Synthase Polyclonal Antibody detects Thymidylate synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thymidylate synthase stained by Thymidylate Synthase Polyclonal Antibody (Product # PA5-27867) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Thymidylate synthetase antibody detects Thymidylate synthetase protein at cytosol and nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Thymidylate synthetase antibody (Product # PA5-27867) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thymidylate synthetase antibody detects Thymidylate synthetase protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Thymidylate synthetase antibody (Product # PA5-27867) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Thymidylate synthetase antibody immunoprecipitates TYMS protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 µg whole cell lysate/extract of TYMS protein expressing Jurkat cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of TYMS by 3 µg of thymidylate synthetase antibody (Product # PA5-27867) 10% SDS-PAGE The immunoprecipitated TYMS protein was detected by thymidylate synthetase antibody (Product # PA5-27867) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.