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LearnNBP1-46823
antibody from Novus Biologicals
Targeting: CDC42BPA
FLJ23347, KIAA0451, MRCK, MRCKA, PK428
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunoprecipitation [2]
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- Product number
- NBP1-46823 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46823, RRID:AB_10009886
- Product name
- Rabbit Polyclonal MRCK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MRCK Antibody [NBP1-46823] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MRCK antibody NBP1-46823 used for IP at 3 ug/mg lysate. MRCK was also immunoprecipitated by rabbit anti-MRCK antibody NBP1-46822, which recognizes an upstream epitope. For blotting immunoprecipitated MRC, NBP1-46822 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MRCK Antibody [NBP1-46823] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TORC3 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TORC3 was also immunoprecipitated by rabbit anti-TORC3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
