MAB1820

antibody from Novus Biologicals

Targeting: S100B S100beta

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1820

Provider

Novus Biologicals

Product name

Mouse Monoclonal S100B Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human S100B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) S100A1, 2, 6, 7, 10, 11, 13, 16, rmS100A4, 8, 9, or rhS100P.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Increased serum S100B levels in chronic schizophrenic patients on long-term clozapine or typical antipsychotics.

Qi LY, Xiu MH, Chen DC, Wang F, Kosten TA, Kosten TR, Zhang XY
Neuroscience letters 2009 Sep 22;462(2):113-7

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human S100B by Western Blot. Western blot shows lysate of human brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB1820) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100B at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human S100B by Simple WesternTM. Detection of Human S100B by Simple WesternTM.