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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- V3423 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- S100B Antibody Cocktail
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 4C4.9 + S100B/1012
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the S100B antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- (Left) Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100 antibody (green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Right) Negative control.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human melanoma with S100B antibody (4C4.9 + S100B/1012). Required HIER: boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
