Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-20376 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Presenilin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human brain tissue lysate.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human brain lysate using a Presenilin1 polyclonal antibody (Product # PA5-20376) at (A) 0.5, (B) 1, and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of presenilin1 in human brain lysate with Presenilin 1 Polyclonal Antibody (Product # PA5-20376) at (A) 0.5, (B) 1, and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Presenilin 1 was achieved by transfecting T-47D with IRF3 specific siRNAs (Silencer® select Product # s112). Western blot analysis (Fig. a) was performed using whole cell extracts from the Presenilin 1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Presenilin 1 Polyclonal Antibody (Product # PA5-20376, 2ug/ml) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b and Fig. c). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Presenilin 1 and C-terminal fragment of Presenilin 1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Presenilin 1 Polyclonal Antibody (Product # PA5-20376) and a 53 kDa band corresponding to Presenilin 1 and ~20 kDa band corresponding to C-terminal fragment of Presenilin 1 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of T-47D (Lane 1), MCF7 (Lane 2), HT-29 (Lane 3), SH-SY5Y (Lane 4) and Daudi (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of Presenilin1 in Human Brain cells with Presenilin 1 Polyclonal Antibody (Product # PA5-20376) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of Presenilin1 in Human Brain cells with Presenilin 1 Polyclonal Antibody (Product # PA5-20376) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of Presenilin1 in human brain tissue with Presenilin 1 Polyclonal Antibody (Product # PA5-20376) at 2.5 µg/mL.
