Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30585 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Presenilin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa.
- Concentration
- 0.53 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Presenilin 1 using 30 µg of HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Presenilin 1 polyclonal antibody (Product # PA5-30585) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Presenilin 1 was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: Presenilin 1 Polyclonal Antibody (Product # PA5 30585) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Presenilin 1 (Product # PA5-30585) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Presenilin 1 Polyclonal Antibody detects PSEN1 protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Presenilin 1 Polyclonal Antibody (Product # PA5-30585) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Presenilin 1 Polyclonal Antibody detects PSEN1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Presenilin 1 Polyclonal Antibody (Product # PA5-30585) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
