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LearnMA5-42820
antibody from Invitrogen Antibodies
Targeting: C4A
C4, C4A2, C4A3, C4A4, C4A6, C4B, C4S, CO4, CPAMD2, RG
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-42820 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Complement C4a Recombinant Rabbit Monoclonal Antibody (4C3F2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: HepG2. Immunogen sequence: HREELVYELN PLDHRGRTLE IPGNSDPNMI PDGDFNSYVR VTASDPLDTL GSEGALSPGG VASLLRLPRG CGEQTMIYLA PTLAASRYLD KTEQWSTLPP ETKDHAVDLI QKGYMRIQQF RKADGSYAAW LSRDSSTWLT AFVLKVLSLA QEQVGGSPEK LQETSNWLLS QQQADGSFQD PCPVLDRSMQ GGLVGNDETV ALTAFVTIAL HHGLAVFQDE GAEPLKQRVE ASISKANSFL GEKASAGLLG AHAAAITAYA LTLTKAPVDL LGVAHNNLMA MAQETGDNLY WGSVTGSQ
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 4C3F2
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Complement C4 in extracts of HepG2 cells. Samples were incubated with Complement C4 Monoclonal antibody (Product # MA5-42820) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Immunofluorescence) analysis of Complement C4 in human liver. Samples were incubated with Complement C4 Monoclonal antibody (Product # MA5-42820) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
