bs-0523R

antibody from Bioss

Targeting: SOX2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

bs-0523R

Provider

Bioss

Proper citation

Bioss Cat#bs-0523R, RRID:AB_10856353

Product name

Rabbit Anti-SOX2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

SOX2

Description

Purified by Protein A.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100ul

Concentration

1ug/ul

Storage

Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

References

Submitted references

Anti-inflammatory effects of quercetin in a mouse model of MC903-induced atopic dermatitis.

Hou DD, Zhang W, Gao YL, Sun YZ, Wang HX, Qi RQ, Chen HD, Gao XH
International immunopharmacology 2019 Sep;74:105676

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Renal proximal tubular epithelial cells exert immunomodulatory function by driving inflammatory CD4⁺ T cell responses.

Breda PC, Wiech T, Meyer-Schwesinger C, Grahammer F, Huber T, Panzer U, Tiegs G, Neumann K
American journal of physiology. Renal physiology 2019 Jul 1;317(1):F77-F89

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rLj-RGD3 induces apoptosis via the mitochondrial-dependent pathway and inhibits adhesion, migration and invasion of human HeyA8 cells via FAK pathway.

Jiang Q, Li Q, Han J, Gou M, Zheng Y, Li B, Xiao R, Wang J
International journal of biological macromolecules 2017 Mar;96:652-668

---

Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice.

Bi J, Zhang S, Du Z, Zhang J, Deng Y, Liu C, Zhang J
Scientific reports 2017 Aug 30;7(1):10001

---

Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis.

Wang Y, Zhao Y, Yu S, Feng Y, Zhang H, Kou X, Chu M, Cui L, Li L, Zhang P, Shen W, Min L
Toxicology and applied pharmacology 2016 Dec 15;313:149-158

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A signature for induced pluripotent stem cell-associated genes in colorectal cancer.

Liu YH, Li Y, Liu XH, Sui HM, Liu YX, Xiao ZQ, Zheng P, Chen L, Yao S, Xing C, Zhou J, Li JM
Medical oncology (Northwood, London, England) 2013 Mar;30(1):426

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Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs.

Gao Q, Zhao L, Song Z, Yang G
Molecular biology reports 2012 May;39(5):5791-804

Western blot

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Lane 1: mouse embryo lysates Lane 2: mouse lung lysates probed with Anti SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 32kD. Observed band size: 32kD.

Sample type

Mouse

Other comments

Embryo

Immunohistochemistry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded human stomach labeled with Anti-SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Sample type

Human

Other comments

Stomach

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded rat esophagus labeled with Anti-SOX2 Polyclonal Antibody, Unconjugated (bs-0523R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Sample type

Rat

Other comments

Esophagus

Flow cytometry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

NCCIT cells probed with Rabbit Anti-SOX2 Polyclonal Antibody (bs-0523R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.

Sample type

Human