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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [4]
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- Product number
- MA1-014-D488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX2 Monoclonal Antibody (20G5), DyLight™ 488
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA1-014-D488 has been successfully used in ICC/IF applications with human and mouse samples.
- Conjugate
- Green dye
- Antibody clone number
- 20G5
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Imunofluorescent analysis of SOX2 (green) in Human episomal iPSC cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with Dylight 488-conjugated SOX2 monoclonal antibody (right, Product # MA1-014-D488) or Dylight 488-conjugated Mouse IgG1 isotype control antibody (left, Product # MA1-191-D488), at a dilution of 1:50 and incubated for 1 hour at room temperature. Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken at 20X magnification.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SOX2 (green) showing nuclear staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated SOX2 monoclonal antibody (Product # MA1-014-D488) in 3% BSA-PBS at a dilution of 1:100 and incubated for 1 hour at 37C in the dark. F-Actin (left panel, red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (left panel, blue) were stained with DAPI. Images were taken at 60X magnification.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Imunofluorescent analysis of SOX2 (green) in Human episomal iPSC cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with Dylight 488-conjugated SOX2 monoclonal antibody (right, Product # MA1-014-D488) or Dylight 488-conjugated Mouse IgG1 isotype control antibody (left, Product # MA1-191-D488), at a dilution of 1:50 and incubated for 1 hour at room temperature. Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken at 20X magnification.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SOX2 (green) shows nuclear staining in human HEL 11.4 iPS cells, indicating pluripotency. SOX2 staining (left panel), DAPI staining (middle panel) and an overlay image of SOX2 with DAPI (right panel) are shown. HEL 11.4 iPS cells were grown on matrigel-coated chamber slides and fixed with 4% formaldehyde for 15 minutes prior to staining. Cells were probed with a DyLight 488-conjugated SOX2 monoclonal antibody (Product # MA1-014-D488) in 0.1% Tween-PBS at a dilution of 1:20, and incubated overnight at 4C. Images were taken at 20X magnification.
- Conjugate
- Green dye
