MA1-014-D650

antibody from Invitrogen Antibodies

Targeting: SOX2

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-014-D650

Provider

Invitrogen Antibodies

Product name

SOX2 Monoclonal Antibody (20G5), DyLight™ 650

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA1-014-D650 has been successfully used in ICC/IF applications with human and mouse samples. MA1-014-D650 can be used for immunofluorescence analysis of LIN28 in human iPSCs.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Antibody clone number

20G5

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C, do not freeze

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Imunofluorescent analysis of SOX2 (red) in Human episomal iPSC cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with Dylight 650-conjugated SOX2 monoclonal antibody (right, Product # MA1-014-D650) or Dylight 650-conjugated Mouse IgG1 isotype control antibody (left, Product # MA1-191-D650), at a dilution of 1:50 and incubated for 1 hour at room temperature. Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken at 20X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SOX2 (red) showing nuclear staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 650-conjugated SOX2 monoclonal antibody (Product # MA1-014-D650) in 3% BSA-PBS at a dilution of 1:50 and incubated for 1 hour at 37C in the dark. Nuclei (left panel, blue) were stained with DAPI. Images were taken at 60X magnification.