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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab128778 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant SOX2 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human SOX2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500 Ð 1/1000IHC: 1/100-1/200ICC/IF: 1/50-1/200Protein FunctionTranscription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-68- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant SOX2 Antibody (Ab128778) - Western Blot All lanes: Recombinant SOX2 Antibody (Ab128778) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 34 kDa Observed band size: 28, 37, 59 kDa Exposure time: 7.8 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant SOX2 Antibody (Ab128778) - Western Blot All lanes: Recombinant SOX2 Antibody (Ab128778) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 9.9 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant SOX2 Antibody (Ab128778) - ICC/IF Immunofluorescence analysis of Recombinant SOX2 Antibody (Ab128778) in NCCIT cells using SOX2 antibody(green), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant SOX2 Antibody (Ab128778) - IHC Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma using Recombinant SOX2 Antibody (Ab128778). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
