MA5-15734

antibody from Invitrogen Antibodies

Targeting: SOX2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15734

Provider

Invitrogen Antibodies

Product name

SOX2 Monoclonal Antibody (10F10)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15734 targets SOX2 in IF, IHC, and WB applications and shows reactivity with Human samples.

Antibody clone number

10F10

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SOX2 using SOX2 monoclonal antibody (Product # MA5-15734) in HEK293 (1) and SOX2 (AA: 2-317) human IgG Fc transfected HEK293 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of SOX2 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Mouse Monoclonal Antibody (Product # MA5-15734) at 1:250 dilution in 0.1% BSA,incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows SOX2 negative cell line HeLa with no signal. Panel f represents control cells with no primary control to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NTERA-2 cells using SOX2 monoclonal antibody (Product # MA5-15734) (Green). Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NTERA-2 cells using SOX2 monoclonal antibody (Product # MA5-15734) (Green). Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and esophageal cancer tissues (right) using SOX2 monoclonal antibody (Product # MA5-15734) followed with DAB staining.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Enrichment of endogenous SOX2 protein at specific gene loci using Anti-SOX2 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-SOX2 Mouse Monoclonal Antibody (Product # MA5-15734, 8 ul) on sheared chromatin from 2 million NTERA-2 cells using the MAGnify ChIP System (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over OCT4 promoter (active) and SAT2 satellite repeats and SAT alpha (inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.