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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- GTX627405 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX627405, RRID:AB_11165499
- Product name
- SOX2 antibody [GT1352]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- SOX2 antibody [GT1352] detects SOX2 protein by western blot analysis.A. 30 ?g human ESC whole cell lysate/extract10% SDS-PAGESOX2 antibody [GT1352] (GTX627405) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOX2 antibody [GT1352] detects SOX2 protein by western blot analysis.A. 30 ?g mouse ESC whole cell lysate/extract10% SDS-PAGESOX2 antibody [GT1352] (GTX627405) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE GTX627405 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 £gg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX627405) diluted at 1:5000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) NT2D1 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.*The competitor is not affiliated with GeneTex and does not endorse this product.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) NT2D1 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOX2 antibody detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Red: SOX2 protein stained by SOX2 antibody (GTX627405) diluted at 1:200.Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- SOX2 antibody [GT1352] detects SOX2 protein at nucleus on by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. SOX2 antibody [GT1352] (GTX627405) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Green: GFAP antibody (GTX108711) diluted at 1:1000. Red: Sox2 antibody [GT1352] (GTX627405) diluted at 1:250.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOX2 antibody [GT1352] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 mouse embryo.Green: Brachyury stained by Brachyury antibody (GTX133714) diluted at 1:250.Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- SOX2 antibody [GT1352] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1352] dilution: 1:50
