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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-47065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TWEAK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) BAFF, rhFas Ligand, rhGITR Ligand, rhOX40 Ligand, rhTRAIL and less than 1% cross-reactivity with rhAPRIL, rhLIGHT, rhTNF-alpha, rhVEGI, and rhTRANCE is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TWEAK in immersion fixed MDA-MB-231 human breast cancer cell line. Samples were incubated in TWEAK polyclonal antibody (Product # PA5-47065) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of TWEAK in HT‚29 human colon adenocarcinoma cell line. Samples were incubated in TWEAK polyclonal antibody (Product # PA5-47065). Recombinant Human TWEAK/TNFSF12 inhibits proliferation in the HT‚29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human TWEAK/TNFSF12 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TWEAK/TNFSF12 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 2-8 µg/mL.
