Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-32282 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal CD3 epsilon Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD3 epsilon Antibody [NBP2-32282] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CD3 epsilon Antibody [NBP2-32282] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for IP at 6 ug per reaction. CD3E was also immunoprecipitated by rabbit anti-CD3E antibody BL16032. For blotting immunoprecipitated CD3E, NBP2-32282 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CD3 epsilon Antibody [NBP2-32282] - Detection of human CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody NBP2-32282 used for IP at 6 ug per reaction. CD3E was also immunoprecipitated by rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (NBP2076424) and another rabbit anti-CD3E antibody. For blotting immunoprecipitated CD3E, NBP2-32282 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD3 epsilon Antibody [NBP2-32282] - Sample: FFPE section of human lung cancer infiltrate. Antibody: Affinity purified rabbit anti- CD3E used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
