PB9093

antibody from Boster Biological Technology

Targeting: CD3E

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PB9093

Provider

Boster Biological Technology

Product name

Anti-CD3 epsilon/CD3E Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for CD3/CD3E detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IHC-F, ICC, IF. Reactive species: Human;Mouse;Rat;Chicken. CD3/CD3E information: Molecular Weight: 23147 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein.

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

IL-33 Contributes to the Pathological Changes of Hair Follicles in Psoriasis: A Potential Target for Psoriatic Alopecia.

Dai C, Chen H, Jiao M, Zhang N, Tang X, Fan A, Liu S, Qian Z, Wang C, Xu Y, Tan Z, Zeng F, Zheng F
Clinical, cosmetic and investigational dermatology 2023;16:639-650

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The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis.

Qu Y, Yang H, Li S, Li L, Li Y, Wang D
International journal of biological macromolecules 2023 May 15;237:123927

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CXCR4 knockout induces neuropathological changes in the MPTP-lesioned model of Parkinson's disease.

Ma J, Dong L, Chang Q, Chen S, Zheng J, Li D, Wu S, Yang H, Li X
Biochimica et biophysica acta. Molecular basis of disease 2023 Feb;1869(2):166597

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An acquired phosphatidylinositol 4-phosphate transport initiates T-cell deterioration and leukemogenesis.

Zhong W, Lin W, Yang Y, Chen D, Cao X, Xu M, Pan G, Chen H, Zheng J, Feng X, Yang LH, Lai C, Olkkonen VM, Xu J, Cui S, Yan D
Nature communications 2022 Jul 29;13(1):4390

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Combination of Chidamide-Mediated Epigenetic Modulation with Immunotherapy: Boosting Tumor Immunogenicity and Response to PD-1/PD-L1 Blockade.

Tu K, Yu Y, Wang Y, Yang T, Hu Q, Qin X, Tu J, Yang C, Kong L, Zhang Z
ACS applied materials & interfaces 2021 Aug 25;13(33):39003-39017

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Dual-Wavelength Photosensitive Nano-in-Micro Scaffold Regulates Innate and Adaptive Immune Responses for Osteogenesis.

Zhao Q, Shi M, Yin C, Zhao Z, Zhang J, Wang J, Shen K, Zhang L, Tang H, Xiao Y, Zhang Y
Nano-micro letters 2020 Nov 21;13(1):28

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of CD3 Epsilon using anti-CD3 Epsilon antibody (PB9093). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human CD3 Epsilon protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3 Epsilon antigen affinity purified polyclonal antibody (Catalog # PB9093) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3 Epsilon at approximately 40KD. The expected band size for CD3 Epsilon is at 40KD.

Additional image