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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [12]
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- Product number
- LS-C343957 - Provider product page
- Provider
- LSBio
- Product name
- CD3E Antibody (aa23-207) LS-C343957
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody Western blot. All lanes: Anti CD3 Epsilon at 0.5 ug/ml. WB : Recombinant Human CD3epsilon Protein 0.5ng . Predicted band size: 40 kD. Observed band size: 40 kD.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody Western blot. All lanes: Anti CD3 Epsilon at 0.5 ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40 ug. Lane 2: CEM Whole Cell Lysate at 40 ug. Predicted band size: 23 kD. Observed band size: 23 kD.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody IHC-paraffin: Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody IHC-paraffin: Mouse Spleen Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody IHC-paraffin: Rat Spleen Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody IHC-frozen: Mouse Spleen Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody. CD3 Epsilon was detected in frozen section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD3 Epsilon Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody IHC-paraffin: Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody IHC-frozen: Rat Spleen Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody IHC-paraffin: Human Tonsil Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody IHC-paraffin: Mouse Spleen Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody IHC-paraffin: Rat Spleen Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CD3 epsilon antibody IHC-frozen: Mouse Spleen Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody. CD3 Epsilon was detected in frozen section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CD3 Epsilon Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
