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Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Other assay [1]
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- Product number
- MAB484-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD3 epsilon Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CD3 epsilon . Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters.1 Binds to the CD3 epsilon-chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.3,4
- Reactivity
- Mouse
- Host
- Hamster
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 145-2C11
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references High-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45+DDR2+ Cells.
IL-23R Signaling Plays No Role in Myocardial Infarction.
Filamin A Is Required for Optimal T Cell Integrin-Mediated Force Transmission, Flow Adhesion, and T Cell Trafficking.
Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis.
mTORC2 controls Th9 polarization and allergic airway inflammation.
Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking.
Protection of CD8+ T cells from activation-induced cell death by IL-18.
Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay.
Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration.
Sidles SJ, Xiong Y, Young MRI, LaRue AC
Mediators of inflammation 2019;2019:1648614
Mediators of inflammation 2019;2019:1648614
IL-23R Signaling Plays No Role in Myocardial Infarction.
Engelowski E, Modares NF, Gorressen S, Bouvain P, Semmler D, Alter C, Ding Z, Flögel U, Schrader J, Xu H, Lang PA, Fischer J, Floss DM, Scheller J
Scientific reports 2018 Nov 20;8(1):17078
Scientific reports 2018 Nov 20;8(1):17078
Filamin A Is Required for Optimal T Cell Integrin-Mediated Force Transmission, Flow Adhesion, and T Cell Trafficking.
Savinko T, Guenther C, Uotila LM, Llort Asens M, Yao S, Tojkander S, Fagerholm SC
Journal of immunology (Baltimore, Md. : 1950) 2018 May 1;200(9):3109-3116
Journal of immunology (Baltimore, Md. : 1950) 2018 May 1;200(9):3109-3116
Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis.
McDonald LT, Johnson SD, Russell DL, Young MRI, LaRue AC
PloS one 2017;12(7):e0180724
PloS one 2017;12(7):e0180724
mTORC2 controls Th9 polarization and allergic airway inflammation.
Chen H, Zhang L, Wang P, Su H, Wang W, Chu Z, Zhang L, Zhang X, Zhao Y
Allergy 2017 Oct;72(10):1510-1520
Allergy 2017 Oct;72(10):1510-1520
Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):409-19
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):409-19
Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2130-9
Journal of immunology (Baltimore, Md. : 1950) 2010 Feb 15;184(4):2130-9
Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking.
Sinclair LV, Finlay D, Feijoo C, Cornish GH, Gray A, Ager A, Okkenhaug K, Hagenbeek TJ, Spits H, Cantrell DA
Nature immunology 2008 May;9(5):513-21
Nature immunology 2008 May;9(5):513-21
Protection of CD8+ T cells from activation-induced cell death by IL-18.
Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H
Journal of leukocyte biology 2007 Jul;82(1):142-51
Journal of leukocyte biology 2007 Jul;82(1):142-51
Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay.
Ogilvie RL, Abelson M, Hau HH, Vlasova I, Blackshear PJ, Bohjanen PR
Journal of immunology (Baltimore, Md. : 1950) 2005 Jan 15;174(2):953-61
Journal of immunology (Baltimore, Md. : 1950) 2005 Jan 15;174(2):953-61
Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration.
Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Jul 7;24(27):6133-43
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Jul 7;24(27):6133-43
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Mouse CD3 epsilon Antibody Stimulates Cell Proliferation in Mouse T Cells. Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) stimulates cell proliferation in mouse T cells in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 0.1-0.6 μg/mL.
