Antibody data
- Antibody Data
- Antigen structure
- References [42]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB100-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD3 epsilon Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Recognizes the epsilon-chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- UCHT1
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL15-Based Trifunctional Antibody-Fusion Proteins with Costimulatory TNF-Superfamily Ligands in the Single-Chain Format for Cancer Immunotherapy.
89Zr-atezolizumab imaging as a non-invasive approach to assess clinical response to PD-L1 blockade in cancer.
Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts.
Interleukin-10-producing LAG3+ regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis.
PLCε1 regulates SDF-1α-induced lymphocyte adhesion and migration to sites of inflammation.
Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue.
Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2.
Human trophoblast cells induced MDSCs from peripheral blood CD14(+) myelomonocytic cells via elevated levels of CCL2.
T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines.
PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation.
Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation.
Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif.
Autologous tumor lysate-pulsed dendritic cell immunotherapy with cytokine-induced killer cells improves survival in gastric and colorectal cancer patients.
1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients.
IκB kinase ε targets interferon regulatory factor 1 in activated T lymphocytes.
CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
The secretion of IL-6 by CpG-ODN-treated cancer cells promotes T-cell immune responses partly through the TLR-9/AP-1 pathway in oral squamous cell carcinoma.
Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation.
A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1.
Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.
Regulated capture by exosomes of mRNAs for cytoplasmic tRNA synthetases.
Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
Phase I therapeutic trial of the HIV-1 Tat protein and long term follow-up.
Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells.
Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3.
Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
"Cytokine storm" in the phase I trial of monoclonal antibody TGN1412: better understanding the causes to improve preclinical testing of immunotherapeutics.
Preferential migration of T regulatory cells induced by IL-16.
B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease.
A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood.
Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis.
Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay.
HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis.
IL-22 increases the innate immunity of tissues.
In vitro differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression.
NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
Beha N, Harder M, Ring S, Kontermann RE, Müller D
Molecular cancer therapeutics 2019 Jul;18(7):1278-1288
Molecular cancer therapeutics 2019 Jul;18(7):1278-1288
89Zr-atezolizumab imaging as a non-invasive approach to assess clinical response to PD-L1 blockade in cancer.
Bensch F, van der Veen EL, Lub-de Hooge MN, Jorritsma-Smit A, Boellaard R, Kok IC, Oosting SF, Schröder CP, Hiltermann TJN, van der Wekken AJ, Groen HJM, Kwee TC, Elias SG, Gietema JA, Bohorquez SS, de Crespigny A, Williams SP, Mancao C, Brouwers AH, Fine BM, de Vries EGE
Nature medicine 2018 Dec;24(12):1852-1858
Nature medicine 2018 Dec;24(12):1852-1858
Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts.
Junker S, Frommer KW, Krumbholz G, Tsiklauri L, Gerstberger R, Rehart S, Steinmeyer J, Rickert M, Wenisch S, Schett G, Müller-Ladner U, Neumann E
Matrix biology : journal of the International Society for Matrix Biology 2017 Oct;62:75-91
Matrix biology : journal of the International Society for Matrix Biology 2017 Oct;62:75-91
Interleukin-10-producing LAG3+ regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis.
Nakachi S, Sumitomo S, Tsuchida Y, Tsuchiya H, Kono M, Kato R, Sakurai K, Hanata N, Nagafuchi Y, Tateishi S, Kanda H, Okamura T, Yamamoto K, Fujio K
Arthritis research & therapy 2017 May 16;19(1):97
Arthritis research & therapy 2017 May 16;19(1):97
PLCε1 regulates SDF-1α-induced lymphocyte adhesion and migration to sites of inflammation.
Strazza M, Azoulay-Alfaguter I, Peled M, Smrcka AV, Skolnik EY, Srivastava S, Mor A
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 7;114(10):2693-2698
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 7;114(10):2693-2698
Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue.
Caër C, Rouault C, Le Roy T, Poitou C, Aron-Wisnewsky J, Torcivia A, Bichet JC, Clément K, Guerre-Millo M, André S
Scientific reports 2017 Jun 7;7(1):3000
Scientific reports 2017 Jun 7;7(1):3000
Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2.
Fischer R, Marsal J, Guttà C, Eisler SA, Peters N, Bethea JR, Pfizenmaier K, Kontermann RE
Scientific reports 2017 Jul 26;7(1):6607
Scientific reports 2017 Jul 26;7(1):6607
Human trophoblast cells induced MDSCs from peripheral blood CD14(+) myelomonocytic cells via elevated levels of CCL2.
Zhang Y, Qu D, Sun J, Zhao L, Wang Q, Shao Q, Kong B, Zhang Y, Qu X
Cellular & molecular immunology 2016 Sep;13(5):615-27
Cellular & molecular immunology 2016 Sep;13(5):615-27
T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines.
Huang W, Lu C, Wu Y, Ouyang S, Chen Y
Journal of experimental & clinical cancer research : CR 2015 May 21;34:54
Journal of experimental & clinical cancer research : CR 2015 May 21;34:54
PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation.
Patsoukis N, Bardhan K, Chatterjee P, Sari D, Liu B, Bell LN, Karoly ED, Freeman GJ, Petkova V, Seth P, Li L, Boussiotis VA
Nature communications 2015 Mar 26;6:6692
Nature communications 2015 Mar 26;6:6692
Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation.
Moore CS, Cui QL, Warsi NM, Durafourt BA, Zorko N, Owen DR, Antel JP, Bar-Or A
Journal of immunology (Baltimore, Md. : 1950) 2015 Jan 15;194(2):761-72
Journal of immunology (Baltimore, Md. : 1950) 2015 Jan 15;194(2):761-72
Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif.
Fan M, Zhang Y, Huang Z, Liu J, Guo X, Zhang H, Luo H
PloS one 2014;9(9):e108253
PloS one 2014;9(9):e108253
Autologous tumor lysate-pulsed dendritic cell immunotherapy with cytokine-induced killer cells improves survival in gastric and colorectal cancer patients.
Gao D, Li C, Xie X, Zhao P, Wei X, Sun W, Liu HC, Alexandrou AT, Jones J, Zhao R, Li JJ
PloS one 2014;9(4):e93886
PloS one 2014;9(4):e93886
1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients.
Van Belle TL, Vanherwegen AS, Feyaerts D, De Clercq P, Verstuyf A, Korf H, Gysemans C, Mathieu C
PloS one 2014;9(10):e109194
PloS one 2014;9(10):e109194
IκB kinase ε targets interferon regulatory factor 1 in activated T lymphocytes.
Sgarbanti M, Marsili G, Remoli AL, Stellacci E, Mai A, Rotili D, Perrotti E, Acchioni C, Orsatti R, Iraci N, Ferrari M, Borsetti A, Hiscott J, Battistini A
Molecular and cellular biology 2014 Mar;34(6):1054-65
Molecular and cellular biology 2014 Mar;34(6):1054-65
CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
Cell stem cell 2014 Mar 6;14(3):342-56
Cell stem cell 2014 Mar 6;14(3):342-56
The secretion of IL-6 by CpG-ODN-treated cancer cells promotes T-cell immune responses partly through the TLR-9/AP-1 pathway in oral squamous cell carcinoma.
Ruan M, Thorn K, Liu S, Li S, Yang W, Zhang C, Zhang C
International journal of oncology 2014 Jun;44(6):2103-10
International journal of oncology 2014 Jun;44(6):2103-10
Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation.
Zhu L, Huang Z, Stålesen R, Hansson GK, Li N
Journal of thrombosis and haemostasis : JTH 2014 Jul;12(7):1156-65
Journal of thrombosis and haemostasis : JTH 2014 Jul;12(7):1156-65
A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1.
Cevikbas F, Wang X, Akiyama T, Kempkes C, Savinko T, Antal A, Kukova G, Buhl T, Ikoma A, Buddenkotte J, Soumelis V, Feld M, Alenius H, Dillon SR, Carstens E, Homey B, Basbaum A, Steinhoff M
The Journal of allergy and clinical immunology 2014 Feb;133(2):448-60
The Journal of allergy and clinical immunology 2014 Feb;133(2):448-60
Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.
Renand A, Milpied P, Rossignol J, Bruneau J, Lemonnier F, Dussiot M, Coulon S, Hermine O
PloS one 2013;8(12):e85589
PloS one 2013;8(12):e85589
Regulated capture by exosomes of mRNAs for cytoplasmic tRNA synthetases.
Wang F, Xu Z, Zhou J, Lo WS, Lau CF, Nangle LA, Yang XL, Zhang M, Schimmel P
The Journal of biological chemistry 2013 Oct 11;288(41):29223-8
The Journal of biological chemistry 2013 Oct 11;288(41):29223-8
Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):409-19
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):409-19
IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4880-8
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4880-8
Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
Ni Z, Walcheck B
Clinical immunology (Orlando, Fla.) 2009 Nov;133(2):257-64
Clinical immunology (Orlando, Fla.) 2009 Nov;133(2):257-64
Phase I therapeutic trial of the HIV-1 Tat protein and long term follow-up.
Longo O, Tripiciano A, Fiorelli V, Bellino S, Scoglio A, Collacchi B, Alvarez MJ, Francavilla V, Arancio A, Paniccia G, Lazzarin A, Tambussi G, Din CT, Visintini R, Narciso P, Antinori A, D'Offizi G, Giulianelli M, Carta M, Di Carlo A, Palamara G, Giuliani M, Laguardia ME, Monini P, Magnani M, Ensoli F, Ensoli B
Vaccine 2009 May 26;27(25-26):3306-12
Vaccine 2009 May 26;27(25-26):3306-12
Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
Blood 2009 Jun 11;113(24):6120-7
Blood 2009 Jun 11;113(24):6120-7
Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells.
Hirosako S, Goto E, Fujii K, Tsumori K, Hirata N, Tsumura S, Kamohara H, Kohrogi H
Clinical and experimental immunology 2009 Feb;155(2):266-74
Clinical and experimental immunology 2009 Feb;155(2):266-74
Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K
Genes and immunity 2009 Dec;10(8):702-14
Genes and immunity 2009 Dec;10(8):702-14
Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3.
Tran CN, Thacker SG, Louie DM, Oliver J, White PT, Endres JL, Urquhart AG, Chung KC, Fox DA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):2989-98
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):2989-98
Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
Rossol M, Meusch U, Pierer M, Kaltenhäuser S, Häntzschel H, Hauschildt S, Wagner U
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4239-48
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4239-48
"Cytokine storm" in the phase I trial of monoclonal antibody TGN1412: better understanding the causes to improve preclinical testing of immunotherapeutics.
Stebbings R, Findlay L, Edwards C, Eastwood D, Bird C, North D, Mistry Y, Dilger P, Liefooghe E, Cludts I, Fox B, Tarrant G, Robinson J, Meager T, Dolman C, Thorpe SJ, Bristow A, Wadhwa M, Thorpe R, Poole S
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 1;179(5):3325-31
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 1;179(5):3325-31
Preferential migration of T regulatory cells induced by IL-16.
McFadden C, Morgan R, Rahangdale S, Green D, Yamasaki H, Center D, Cruikshank W
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6439-45
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6439-45
B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease.
Kawai T, Matsuyama T, Hosokawa Y, Makihira S, Seki M, Karimbux NY, Goncalves RB, Valverde P, Dibart S, Li YP, Miranda LA, Ernst CW, Izumi Y, Taubman MA
The American journal of pathology 2006 Sep;169(3):987-98
The American journal of pathology 2006 Sep;169(3):987-98
A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood.
Markovic SN, Nevala WK, Uhl CB, Celis E, McKean DJ
Clinical and experimental immunology 2006 Sep;145(3):438-47
Clinical and experimental immunology 2006 Sep;145(3):438-47
Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis.
Mamoni RL, Blotta MH
Cytokine 2006 Aug;35(3-4):207-16
Cytokine 2006 Aug;35(3-4):207-16
Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
Histopathology 2005 Jun;46(6):659-67
Histopathology 2005 Jun;46(6):659-67
Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
Liao TS, Yurgelun MB, Chang SS, Zhang HZ, Murakami K, Blaine TA, Parisien MV, Kim W, Winchester RJ, Lee FY
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2005 Jan;23(1):203-9
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2005 Jan;23(1):203-9
Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay.
Ogilvie RL, Abelson M, Hau HH, Vlasova I, Blackshear PJ, Bohjanen PR
Journal of immunology (Baltimore, Md. : 1950) 2005 Jan 15;174(2):953-61
Journal of immunology (Baltimore, Md. : 1950) 2005 Jan 15;174(2):953-61
HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis.
Janardhan A, Swigut T, Hill B, Myers MP, Skowronski J
PLoS biology 2004 Jan;2(1):E6
PLoS biology 2004 Jan;2(1):E6
IL-22 increases the innate immunity of tissues.
Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
Immunity 2004 Aug;21(2):241-54
Immunity 2004 Aug;21(2):241-54
In vitro differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression.
Takahashi R, Mizukawa Y, Yamazaki Y, Hayakawa K, Hayakawa J, Kudo A, Shiohara T
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):5769-77
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):5769-77
NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
Han MK, Kim JS, Park BH, Kim JR, Hwang BY, Lee HY, Song EK, Yoo WH
Journal of leukocyte biology 2003 Apr;73(4):525-9
Journal of leukocyte biology 2003 Apr;73(4):525-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD3 epsilon in Human PBMCs. CD3 epsilon was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).