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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Immunohistochemistry [3]
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- Product number
- NB600-1441 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1441, RRID:AB_789102
- Product name
- Rabbit Monoclonal CD3 Antibody
- Antibody type
- Monoclonal
- Description
- Tissue culture supernatant. The CD3 antigen is present on early thymocytes and mature T cells and is generally regarded as a pan-T cell marker. This antibody will help detect CD3 expression in normal and neoplastic tissues. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.
- Reactivity
- Human, Mouse, Canine
- Host
- Rabbit
- Antigen sequence
aa 156-168 of epsilon chain of huma
n CD3 protein (intracytoplasmic).- Isotype
- IgG
- Vial size
- 0.5 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Siah2 control of T-regulatory cells limits anti-tumor immunity.
Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes.
Beta Cell Dedifferentiation Induced by IRE1α Deletion Prevents Type 1 Diabetes.
High-Plex Predictive Marker Discovery for Melanoma Immunotherapy-Treated Patients Using Digital Spatial Profiling.
Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β.
Retinal pathology in experimental optic neuritis is characterized by retrograde degeneration and gliosis.
Antitumor Responses in the Absence of Toxicity in Solid Tumors by Targeting B7-H3 via Chimeric Antigen Receptor T Cells.
Exploring the Role of Tertiary Lymphoid Structures Using a Mouse Model of Bacteria-Infected Lungs.
Increased expression of colony-stimulating factor-1 in mouse spinal cord with experimental autoimmune encephalomyelitis correlates with microglial activation and neuronal loss.
89Zr-labeled nivolumab for imaging of T-cell infiltration in a humanized murine model of lung cancer.
Immune Marker Profiling and Programmed Death Ligand 1 Expression Across NSCLC Mutations.
Regulatory T Cells Restrain Pathogenic T Helper Cells during Skin Inflammation.
A dormant TIL phenotype defines non-small cell lung carcinomas sensitive to immune checkpoint blockers.
Objective measurement and clinical significance of IDO1 protein in hormone receptor-positive breast cancer.
Multiplexed quantitative analysis of CD3, CD8, and CD20 predicts response to neoadjuvant chemotherapy in breast cancer.
Scortegagna M, Hockemeyer K, Dolgalev I, Poźniak J, Rambow F, Li Y, Feng Y, Tinoco R, Otero DC, Zhang T, Brown K, Bosenberg M, Bradley LM, Marine JC, Aifantis I, Ronai ZA
Nature communications 2020 Jan 7;11(1):99
Nature communications 2020 Jan 7;11(1):99
Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes.
Fujimoto N, He Y, D'Addio M, Tacconi C, Detmar M, Dieterich LC
PLoS biology 2020 Apr;18(4):e3000704
PLoS biology 2020 Apr;18(4):e3000704
Beta Cell Dedifferentiation Induced by IRE1α Deletion Prevents Type 1 Diabetes.
Lee H, Lee YS, Harenda Q, Pietrzak S, Oktay HZ, Schreiber S, Liao Y, Sonthalia S, Ciecko AE, Chen YG, Keles S, Sridharan R, Engin F
Cell metabolism 2020 Apr 7;31(4):822-836.e5
Cell metabolism 2020 Apr 7;31(4):822-836.e5
High-Plex Predictive Marker Discovery for Melanoma Immunotherapy-Treated Patients Using Digital Spatial Profiling.
Toki MI, Merritt CR, Wong PF, Smithy JW, Kluger HM, Syrigos KN, Ong GT, Warren SE, Beechem JM, Rimm DL
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Sep 15;25(18):5503-5512
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Sep 15;25(18):5503-5512
Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β.
Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, Waisman A, Opitz L, Oukka M, Schreiner B, Pelczar P, Becher B
Immunity 2019 May 21;50(5):1289-1304.e6
Immunity 2019 May 21;50(5):1289-1304.e6
Retinal pathology in experimental optic neuritis is characterized by retrograde degeneration and gliosis.
Manogaran P, Samardzija M, Schad AN, Wicki CA, Walker-Egger C, Rudin M, Grimm C, Schippling S
Acta neuropathologica communications 2019 Jul 17;7(1):116
Acta neuropathologica communications 2019 Jul 17;7(1):116
Antitumor Responses in the Absence of Toxicity in Solid Tumors by Targeting B7-H3 via Chimeric Antigen Receptor T Cells.
Du H, Hirabayashi K, Ahn S, Kren NP, Montgomery SA, Wang X, Tiruthani K, Mirlekar B, Michaud D, Greene K, Herrera SG, Xu Y, Sun C, Chen Y, Ma X, Ferrone CR, Pylayeva-Gupta Y, Yeh JJ, Liu R, Savoldo B, Ferrone S, Dotti G
Cancer cell 2019 Feb 11;35(2):221-237.e8
Cancer cell 2019 Feb 11;35(2):221-237.e8
Exploring the Role of Tertiary Lymphoid Structures Using a Mouse Model of Bacteria-Infected Lungs.
Teillaud JL, Regard L, Martin C, Sibéril S, Burgel PR
Methods in molecular biology (Clifton, N.J.) 2018;1845:223-239
Methods in molecular biology (Clifton, N.J.) 2018;1845:223-239
Increased expression of colony-stimulating factor-1 in mouse spinal cord with experimental autoimmune encephalomyelitis correlates with microglial activation and neuronal loss.
Gushchina S, Pryce G, Yip PK, Wu D, Pallier P, Giovannoni G, Baker D, Bo X
Glia 2018 Oct;66(10):2108-2125
Glia 2018 Oct;66(10):2108-2125
89Zr-labeled nivolumab for imaging of T-cell infiltration in a humanized murine model of lung cancer.
England CG, Jiang D, Ehlerding EB, Rekoske BT, Ellison PA, Hernandez R, Barnhart TE, McNeel DG, Huang P, Cai W
European journal of nuclear medicine and molecular imaging 2018 Jan;45(1):110-120
European journal of nuclear medicine and molecular imaging 2018 Jan;45(1):110-120
Immune Marker Profiling and Programmed Death Ligand 1 Expression Across NSCLC Mutations.
Toki MI, Mani N, Smithy JW, Liu Y, Altan M, Wasserman B, Tuktamyshov R, Schalper K, Syrigos KN, Rimm DL
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2018 Dec;13(12):1884-1896
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2018 Dec;13(12):1884-1896
Regulatory T Cells Restrain Pathogenic T Helper Cells during Skin Inflammation.
Hartwig T, Zwicky P, Schreiner B, Yawalkar N, Cheng P, Navarini A, Dummer R, Flatz L, Conrad C, Schlapbach C, Becher B
Cell reports 2018 Dec 26;25(13):3564-3572.e4
Cell reports 2018 Dec 26;25(13):3564-3572.e4
A dormant TIL phenotype defines non-small cell lung carcinomas sensitive to immune checkpoint blockers.
Gettinger SN, Choi J, Mani N, Sanmamed MF, Datar I, Sowell R, Du VY, Kaftan E, Goldberg S, Dong W, Zelterman D, Politi K, Kavathas P, Kaech S, Yu X, Zhao H, Schlessinger J, Lifton R, Rimm DL, Chen L, Herbst RS, Schalper KA
Nature communications 2018 Aug 10;9(1):3196
Nature communications 2018 Aug 10;9(1):3196
Objective measurement and clinical significance of IDO1 protein in hormone receptor-positive breast cancer.
Carvajal-Hausdorf DE, Mani N, Velcheti V, Schalper KA, Rimm DL
Journal for immunotherapy of cancer 2017 Oct 17;5(1):81
Journal for immunotherapy of cancer 2017 Oct 17;5(1):81
Multiplexed quantitative analysis of CD3, CD8, and CD20 predicts response to neoadjuvant chemotherapy in breast cancer.
Brown JR, Wimberly H, Lannin DR, Nixon C, Rimm DL, Bossuyt V
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Dec 1;20(23):5995-6005
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Dec 1;20(23):5995-6005
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - Formalin fixed paraffin embedded human tonsil stained with CD3 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD3 Antibody (SP7) [NB600-1441] - T-cells and microglia in mouse spinal cord with acute EAE. Mouse was perfused with 4% paraformaldehyde. Spinal cord was post-fixed overnight, followed by cryoprotection with 30% sucrose for 24h. Spinal cord sections were treated with antigen-retrieval buffer (pH 6.0) at 60C for 10 min, then cooled down to room temperature (this step should not be omitted). Anti-CD3 antibody was diluted at 1:200. Incubation: room temperature overnight. This image was submitted via customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - Mouse lung tissue stained against C3aR normal conditions (left) and knockdown conditions (right). This image was submitted via customer review.
