Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- V3287 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD3e Antibody
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- C3e/1308
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) a partial recombinant protein and 2) human Jurkat cell lysate with CD3e antibody (clone C3e/1308). Predicted molecular weight ~23 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human tonsil tissue with CD3e antibody (clone C3e/1308). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone C3e/1308). Confirmation of Integrity and Purity of the Antibody.