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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- V3462 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD3e Antibody / Cytoplasmic domain
- Antibody type
- Monoclonal
- Description
- This highly specific CD3e antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- PC3/188A
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Jurkat cell lysate with CD3e antibody (clone PC3/188A). Expected molecular weight ~23 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human tonsil tissue with CD3e antibody (clone PC3/188A). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free CD3e Antibody (clone PC3/188A). Confirmation of Integrity and Purity of the Antibody.
