SM1754P

antibody from OriGene

Targeting: CD3E

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

SM1754P

Provider

OriGene

Product name

CD3E (Cytopl. Dom.) rat monoclonal antibody, clone CD3-12, Purified

Antibody type

Monoclonal

Description

CD3E (Cytopl. Dom.) rat monoclonal antibody, clone CD3-12, Purified

Host

Rat

Conjugate

Unconjugated

Epitope

CD3E

Isotype

IgG

Antibody clone number

CD3-12

Vial size

200 µg

Concentration

1.0 mg/ml

References

Submitted references

B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes.

Shrestha B, Hashiguchi T, Ito T, Miura N, Takenouchi K, Oyama Y, Kawahara K, Tancharoen S, Ki-I Y, Arimura N, Yoshinaga N, Noma S, Shrestha C, Nitanda T, Kitajima S, Arimura K, Sato M, Sakamoto T, Maruyama I
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4819-26

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of Jurkat?human acute T-cell leukemia whole cell lysate probed with with anti Human CD3 antibody . followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemitry in Formalin-fixed, Paraffin embedded human tonsil stained with CD3 antibody following heat mediated antigen retrieval in EDTA buffer pH 9.0

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Staining of Human peripheral blood lymphocytes (permeabilised) with Rat anti Human CD3 antibody .

Validation comment

FC