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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- NBP2-53386-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD3 epsilon Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Rat, Bovine, Chicken/Avian, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (PC3/188A).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Thymus lysate using Purified CD3 epsilon Antibody (PC3/188A).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (PC3/188A).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Flow Cytometry: CD3 epsilon Antibody (PC3/188A) [Alexa Fluor® 647] [NBP2-54405AF647] - An intracellular stain was performed on Jurkat cells with CD3 epsilon Antibody (PC3/188A) NBP2-54405AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - An intracellular stain was performed on Jurkat cells with CD3 epsilon Antibody (PC3/188A) NBP2-54405AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
